OMIA 000211-9615 : Coat colour, merle in Canis lupus familiaris

Mendelian trait/disorder: yes

Mode of inheritance: Autosomal Incompletely Dominant

Considered a defect: no

Key variant known: yes

Year key variant first reported: 2006

Mapping: Using a genome scan with the "multiplexed Minimal Screening Set 2" of 327 canine microsatellites genotyped on 9 merle and 32 non-merle Shetland Sheepdogs, Clark et al. (2006) LD-mapped the merle locus to a single marker (FH2537) on chromosome CFA10. A search of homologous regions in the genomes of humans and mice revealed the SILV gene as a comparative positional candidate.

Molecular basis: Sequencing of a very likely comparative positional candidate gene (SILV or PMEL17; now known as PMEL) (see Mapping section above) in Shetland Sheedogs of the three merle genotypes by Clark et al. (2006) revealed "an insertion of a tRNA-derived SINE . . . . The insertion occurs at the boundary of intron 10 and exon 11 and is flanked by a 15-bp target site duplication . . . . The SINE insertion is in reverse orientation, with the 5′ end closer to exon 11."

Having noticed in a mixed-breed non-merle dog some ophthalmologic abnormalities similar to those seen in merle dogs, Imamoto et al. (2011) discovered that this mixed-breed dog was actually carrying the above SINE-insertion PMEL merle mutation, and was therefore a cryptic merle.

By investigating merle-related coat colour and length of SINE insertion in 175 dogs, Ballif et al. (2018) concluded that "there is a continuum of merle insertion lengths associated with a spectrum of coat color and patterns and that genotype-phenotype exceptions and overlap make it difficult to strictly assign certain insertion sizes with an expected coat color, although some generalizations are possible".

Murphy et al. (2018) reported that "In fragment analyses from 259 dogs heterozygous for Merle, we observed a spectrum of oligo(dT) lengths spanning 25 to 105 base pairs (bp), with ranges that correspond to the four varieties of the merle phenotype: cryptic (25-55 bp), dilute (66-74 bp), standard (78-86 bp), and harlequin (81-105 bp). Somatic contractions of the oligo(dT) were observed in 43% of standard and 51% of harlequin merle dogs. A small proportion (4.6%) of the study cohort inherited de novo contractions or expansions of the Merle allele that resulted in dilute or harlequin coat patterns, respectively". These authors concluded that "The phenotypic consequence of the Merle SINE insertion directly depends upon oligo(dT) length. In transcription, we propose that the use of an alternative splice site increases with oligo(dT) length, resulting in insufficient PMEL and a pigment dilution spectrum, from dark grey to complete hypopigmentation. We further propose that during replication, contractions and expansions increase in frequency with oligo(dT) length, causing coat variegation (somatic events in melanocytes) and the spontaneous appearance of varieties of the merle phenotype (germline events)".

Clinical features: As reported by Clark et al. (2006)(citing Sorsby and Davey (1954), "Dogs having Mm and MM genotypes typically have blue eyes and often exhibit a wide range of auditory and ophthalmologic abnormalities"

Prevalence: Mizukami et al. (2016) reported the frequency of the SINE-insertion allele as 0.016 in 500 Border collies in Japan.

Breed: Shetland Sheepdog.

Associated gene:

Symbol Description Species Chr Location OMIA gene details page Other Links
PMEL premelanosome protein Canis lupus familiaris 10 NC_006592.3 (327618..292595) PMEL Homologene, Ensembl, NCBI gene

Variants

By default, variants are sorted chronologically by year of publication, to provide a historical perspective.

Readers can re-sort on any column by clicking on the column header. Click it again to sort in a descending order. To create a multiple-field sort, hold down Shift while clicking on the required column headers

Breed(s) Variant Phenotype Gene Allele Type of Variant Reference Sequence Chr. g. or m. c. or n. p. Verbal Description EVA ID Year Published PubMed ID(s) Acknowledgements
Coat colour, merle PMEL insertion, gross (>20) "an insertion of a tRNA-derived SINE . . . . The insertion occurs at the boundary of intron 10 and exon 11 and is flanked by a 15-bp target site duplication . . . . The SINE insertion is in reverse orientation, with the 5' end closer to exon 11." 2006 16407134

References


Note: the references are listed in reverse chronological order (from the most recent year to the earliest year), and alphabetically by first author within a year.
2018 Ballif, B.C., Ramirez, C.J., Carl, C.R., Sundin, K., Krug, M., Zahand, A., Shaffer, L.G., Flores-Smith, H. :
The PMEL Gene and Merle in the Domestic Dog: A Continuum of Insertion Lengths Leads to a Spectrum of Coat Color Variations in Australian Shepherds and Related Breeds. Cytogenet Genome Res :, 2018. Pubmed reference: 30071510. DOI: 10.1159/000491408.
Murphy, S.C., Evans, J.M., Tsai, K.L., Clark, L.A. :
PMEL: correlating genotype with phenotype. Mob DNA 9:26, 2018. Pubmed reference: 30123327. DOI: 10.1186/s13100-018-0131-6.
2016 Mizukami, K., Yabuki, A., Kohyama, M., Kushida, K., Rahman, M.M., Uddin, M.M., Sawa, M., Yamato, O. :
Molecular prevalence of multiple genetic disorders in Border collies in Japan and recommendations for genetic counselling. Vet J 214:21-3, 2016. Pubmed reference: 27387721. DOI: 10.1016/j.tvjl.2016.05.004.
2011 Imamoto, S., Watanabe, M., Iba, M., Imamoto, M., Umeyama, K. :
A mixed-breed dog showing the same gene modification as in a Miniature Dachshund Journal of Animal Clinical Medicine 20:1-5, 2011. DOI: 10.11252/dobutsurinshoigaku.20.1.
2010 Imamoto, S., Imamoto, M., Watanabe, M., Umeyama, K. :
Study on the effect SINE insertion to an SILV (Pmel17) gene on the fundi of longhaired miniature dachshunds Journal of Veterinary Medicine (Japan) 63:385-390, 2010.
2009 Strain, GM., Clark, LA., Wahl, JM., Turner, AE., Murphy, KE. :
Prevalence of deafness in dogs heterozygous or homozygous for the merle allele. J Vet Intern Med 23:282-6, 2009. Pubmed reference: 19192156. DOI: 10.1111/j.1939-1676.2008.0257.x.
2006 Clark, LA., Wahl, JM., Rees, CA., Murphy, KE. :
From The Cover: Retrotransposon insertion in SILV is responsible for merle patterning of the domestic dog. Proc Natl Acad Sci U S A 103:1376-81, 2006. Pubmed reference: 16407134. DOI: 10.1073/pnas.0506940103.
Hedan, B., Corre, S., Hitte, C., Dreano, S., Vilboux, T., Derrien, T., Denis, B., Galibert, F., Galibert, MD., Andre, C. :
Coat colour in dogs: identification of the merle locus in the Australian shepherd breed. BMC Vet Res 2:9, 2006. Pubmed reference: 16504149. DOI: 10.1186/1746-6148-2-9.
Platt, S., Freeman, J., di Stefani, A., Wieczorek, L., Henley, W. :
Prevalence of unilateral and bilateral deafness in border collies and association with phenotype. J Vet Intern Med 20:1355-62, 2006. Pubmed reference: 17186850.
2003 Schmutz, SM., Berryere, TG., Sharp, CA. :
KITLG maps to canine chromosome 15 and is excluded as a candidate gene for merle in dogs. Anim Genet 34:75-6, 2003. Pubmed reference: 12580795.
1988 Herrman, A., Wegner, W. :
[Eye lesions in aging merle Dachshunds with particular reference to iris atrophy] Praktische Tierarzt 69:33-36, 1988.
1986 Klinckmann, G., Koniszewski, G., Wegner, W. :
Light-microscope investigations on the retinae of dogs carrying the Merle factor Journal of Veterinary Medicine. Series A 33:674-788, 1986. Pubmed reference: 3099512.
1985 Sponenberg, D.P. :
Inheritance of the harlequin color in Great Dane dogs Journal of Heredity 76:224-225, 1985. Pubmed reference: 3998446.
Sponenberg, D.P., Lamoreaux, M.L. :
Inheritance of tweed, a modification of merle, in Australian shepherd dogs Journal of Heredity 76:303-304, 1985. Pubmed reference: 4031467.
1984 Sponenberg, D.P. :
Germline reversion of the merle allele in Australian shepherd dogs Journal of Heredity 75:78 only, 1984. Pubmed reference: 6323572.
1983 Akcan, A., Wegner, W. :
[Changes in the visual pathways and visual centers in Merle syndrome in the dog]. Z Versuchstierkd 25:91-9, 1983. Pubmed reference: 6410614.
1982 Whitney III, J.B., Lamoreux, M.L. :
Transposable elements controlling genetic instabilities in mammals. Journal of Heredity 73:12-18, 1982.
1980 Flach, M., Dausch, D., Wegner, W. :
[Fluorescence angiography in the dachshund. Further findings in Merle syndrome of the dog]. Tierarztl Prax 8:375-83, 1980. Pubmed reference: 6817463.
Wegner, W., Akcan, A. :
[Effects of Merle factor on the optic area in the dog]. Dtsch Tierarztl Wochenschr 87:342, 1980. Pubmed reference: 7006993.
1978 Dausch, D., Wegner, W., Michaelis, W., Reetz, I. :
[Eye changes in the merle syndrome in the dog (author's transl)]. Albrecht Von Graefes Arch Klin Exp Ophthalmol 206:135-50, 1978. Pubmed reference: 418699.
1977 Dausch, D., Wegner, W., Michaelis, M., Reetz, I. :
[Ophthalmological findings in Merle dachshunds]. Dtsch Tierarztl Wochenschr 84:468-75, 1977. Pubmed reference: 340189.
Reetz, I., Stecker, M., Wegner, W. :
Audiometric findings in Dachshunds (merle gene carriers) Deutsche Tierarztliche Wochenschrift 84:273-277, 1977. Pubmed reference: 330141.
1954 Sorsby, A., Davey, J.B. :
Ocular associations of dappling (or merling) in the coat colour of dogs. I. Clinical and genetical data Journal of Genetics 52:425-440, 1954.
1953 Ford, L. :
Defective Collie dogs with heterozygous merling Journal of Canine Genetics :24-28, 1953.
1935 Mitchell, A.L. :
Dominant dilution and other colour factors in Collie dogs Journal of Heredity 26:424-430, 1935.

Edit History


  • Created by Frank Nicholas on 30 Sep 2010
  • Changed by Frank Nicholas on 26 Sep 2011
  • Changed by Frank Nicholas on 12 Dec 2011
  • Changed by Frank Nicholas on 05 Dec 2012
  • Changed by Frank Nicholas on 13 Jan 2014
  • Changed by Frank Nicholas on 07 Oct 2016
  • Changed by Frank Nicholas on 18 Oct 2016
  • Changed by Frank Nicholas on 08 Aug 2018
  • Changed by Frank Nicholas on 21 Sep 2018