OMIA:001868-9615 : Eye, blue in Canis lupus familiaris
In other species: rabbit
Categories: Pigmentation phene
Links to MONDO diseases: No links.
Mendelian trait/disorder: unknown
Considered a defect: no
Inheritance: Deane-Coe et al. (2018): "The high proportion of blue-eyed heterozygotes in our analyses (53% of blue-eyed dogs) suggests that the duplication, if causal, is dominant in its phenotypic effect. However, the existence of 46 brown-eyed heterozygotes with similarly elevated Δ log R (P = 0.35 comparing blue and brown heterozygote distributions) suggests that one or more additional genetic factors may modify or mask the duplication’s effect on eye color (Fig 3; S8 Fig). This effect is not completely explained by an individual’s genotype at four previously characterized pigmentation genes (A, E, B, and K loci; S6 Table), although carriers of the duplication that also carry at least one copy of the dominant melanistic mask (Em) allele are significantly more likely to have brown eyes than duplication carriers without melanistic mask (P = 0.0018)."
Mapping: Via a GWAS conducted with "Embark’s custom high-density 214,661-marker platform" on 3,180 dogs comprising many breeds and mixed-breeds, and with binary phenotypic data on blue/brown eye colour gathered from owners by online survey, Deane-Coe et al. (2018) "detected two significant associations with blue eyes, one on chromosome 10 at PMEL17" (the merle locus - see OMIA OMIA 000211-9615) and "a novel association with blue eyes on chromosome 18 (P = 1.3x10-68) and used both sequence coverage and microarray probe intensity data to identify the putative causal variant: a 98.6-kb duplication directly upstream of the Homeobox gene ALX4, which plays an important role in mammalian eye development. This duplication is largely restricted to Siberian Huskies, is strongly associated with the blue-eyed phenotype (chi-square P = 5.2x10-290), and is highly, but not completely, penetrant."
Markers: Deane-Coe et al. (2018): "we discovered a haplotype containing a 98.6-kb duplication that is strongly predictive of blue eyes and heterochromia in dogs. While we cannot definitively rule out a different typed or untyped variant on this haplotype causing the trait, we feel that the duplication is a plausible causal candidate worthy of further functional investigation. . . . we have shown that this mutation is highly (but not completely) penetrant and most common in Siberian Huskies. The presence of the duplication explains at least 75% of blue-eyed cases in our discovery panel of customer dogs (81 / 108 blue-eyed dogs carried the associated haplotype and have elevated Δ log R values consistent with duplicated markers)."
By default, variants are sorted chronologically by year of publication, to provide a historical perspective.
Readers can re-sort on any column by clicking on the column header. Click it again to sort in a descending
order. To create a multiple-field sort, hold down Shift while clicking on the second, third etc relevant column
WARNING! Inclusion of a variant in this table does not automatically mean that it should be used for DNA testing. Anyone contemplating the use of any of these variants for DNA testing should examine critically the relevant evidence (especially in breeds other than the breed in which the variant was first described). If it is decided to proceed, the location and orientation of the variant sequence should be checked very carefully.
Since October 2021, OMIA includes a semiautomated lift-over pipeline to facilitate updates of genomic positions to a recent reference genome position. These changes to genomic positions are not always reflected in the ‘acknowledgements’ or ‘verbal description’ fields in this table.
|OMIA Variant ID||Breed(s)||Variant Phenotype||Gene||Allele||Type of Variant||Source of Genetic Variant||Reference Sequence||Chr.||g. or m.||c. or n.||p.||Verbal Description||EVA ID||Inferred EVA rsID||Year Published||PubMed ID(s)||Acknowledgements|
|1129||Siberian Husky||Blue eyes||ALX4||duplication||Naturally occurring variant||CanFam3.1||18||g.44791414_44890185dup||Deane-Coe et al. (2018): "a 98.6-kb duplication directly upstream of the Homeobox gene ALX4"||2018||30286082||g. coordinates kindly provided by Anna Letko and Cord Drögemüller (7th Oct 2019)|
|2018||Deane-Coe, P.E., Chu, E.T., Slavney, A., Boyko, A.R., Sams, A.J. :|
|Direct-to-consumer DNA testing of 6,000 dogs reveals 98.6-kb duplication associated with blue eyes and heterochromia in Siberian Huskies. PLoS Genet 14:e1007648, 2018. Pubmed reference: 30286082 . DOI: 10.1371/journal.pgen.1007648.|
- Changed by Frank Nicholas on 27 Feb 2019
- Created by Frank Nicholas on 27 Feb 2019
- Changed by Frank Nicholas on 07 Oct 2019
- Changed by Frank Nicholas on 12 Jun 2020