OMIA:002436-9823 : Digestive enzyme deficiency in Sus scrofa (pig)
Categories: Normal phene
Links to MONDO diseases: No links.
Mendelian trait/disorder: unknown
Considered a defect: no
Species-specific description: Wang et al. (2020): "Unlike ruminants, pigs lack several digestive enzymes such as pectinase, xylanase, cellulase, β-1.3-1.4-glucanase, and phytase which are essential to hydrolyze the cell walls of grains to release endocellular nutrients into their digestive tracts. we synthesized multiple cellulase and pectinase genes derived from lower organisms and then codon-optimized these genes to be expressed in pigs. These genes were then cloned into our previously optimized XynB (xylanase)- EsAPPA (phytase) bicistronic construct. We then successfully generated transgenic pigs that expressed the four enzymes [Pg7fn (pectinase), XynB (xylanase), EsAPPA (phytase), and TeEGI (cellulase and β-glucanase)] using somatic cell cloning. The expression of these genes was parotid gland specific. " This study involves genetically modified organisms (GMO).
Have human generated variants been created, e.g. through genetic engineering and gene editing
Cite this entry
|2020||Wang, H., Li, G., Zhong, C., Mo, J., Sun, Y., Shi, J., Zhou, R., Li, Z., Wu, Z., Liu, D., Zhang, X. :|
|Generation of multi-transgenic pigs using PiggyBac transposons co-expressing pectinase, xylanase, cellulase, β-1.3-1.4-glucanase and phytase. Front Genet 11:597841, 2020. Pubmed reference: 33329743. DOI: 10.3389/fgene.2020.597841.|
- Created by Imke Tammen2 on 27 Sep 2021
- Changed by Imke Tammen2 on 27 Sep 2021