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OMIA:002497-9823 : Resistance/susceptibility to African swine fever virus (ASFV) in Sus scrofa (pig)

In other species: Common warthog

Categories: Immune system phene

Links to possible relevant human trait(s) and/or gene(s) in OMIM: 164014 (gene)

Mendelian trait/disorder: no

Disease-related: yes

Key variant known: no

Species-specific description: This phene includes references to studies involving gene edited or genetically modified organisms (GMO).

Molecular basis: Zheng et al. (2024) "explore the potential of a multiplexed CRISPR-Cas system in suppressing ASFV replication and infection. By engineering CRISPR-Cas systems to target nine specific loci within the ASFV genome, we observed a substantial reduction in viral replication in vitro. ... To further evaluate its anti-viral function in vivo, we developed a pig strain expressing the multiplexable CRISPR-Cas-gRNA via germline genome editing. These transgenic pigs exhibited normal health with continuous expression of the CRISPR-Cas-gRNA system, and a subset displayed latent viral replication and delayed infection. However, the CRISPR-Cas9-engineered pigs did not exhibit a survival advantage upon exposure to ASFV." 
Li et al. (2024) "used the African swine fever virus (ASFV) as an example to investigate the effect of deleting the TRBV27-encoded CDR1 on the resistance of domestic pigs to exotic pathogens. ... The TRBV-edited and wild-type pigs were selected for synchronous ASFV infection. White blood cells were significantly reduced in the genetically modified pigs before ASFV infection. The genetically modified and wild-type pigs were susceptible to ASFV and exhibited typical fevers (>40 °C). However, the TRBV27-edited pigs had a higher viral load than the wild-type pigs. Consistent with this, the gene-edited pigs showed more clinical signs than the wild-type pigs. In addition, both groups of pigs died within 10 days and showed similar severe lesions in organs and tissues."

Genetic engineering: Yes - in addition to the occurrence of natural variants, variants have been created artificially, e.g. by genetic engineering or gene editing
Have human generated variants been created, e.g. through genetic engineering and gene editing

Cite this entry

Nicholas, F. W., Tammen, I., & Sydney Informatics Hub. (2024). OMIA:002497-9823: Online Mendelian Inheritance in Animals (OMIA) [dataset]. https://omia.org/. https://doi.org/10.25910/2AMR-PV70

References

Note: the references are listed in reverse chronological order (from the most recent year to the earliest year), and alphabetically by first author within a year.

2024 Banabazi, M.H., Freimanis, G., Goatley, L.C., Netherton, C.L., de Koning, D.J. :
The transcriptomic insight into the differential susceptibility of African Swine Fever in inbred pigs. Sci Rep 14:5944, 2024. Pubmed reference: 38467747. DOI: 10.1038/s41598-024-56569-2.
Li, J., Xing, H., Liu, K., Fan, N., Xu, K., Zhao, H., Jiao, D., Wei, T., Cheng, W., Guo, J., Zhang, X., Zhu, F., Bu, Z., Zhao, D., Wang, W., Wei, H.J. :
Dysfunction of complementarity determining region 1 encoded by T cell receptor beta variable gene is potentially associated with African swine fever virus infection in pigs. Microorganisms 12:1113, 2024. Pubmed reference: 38930494. DOI: 10.3390/microorganisms12061113.
Qi, F., Chen, X., Wang, J., Niu, X., Li, S., Huang, S., Ran, X. :
Genome-wide characterization of structure variations in the Xiang pig for genetic resistance to African swine fever. Virulence 15:2382762, 2024. Pubmed reference: 39092797. DOI: 10.1080/21505594.2024.2382762.
Shen, D., Zhang, G., Weng, X., Liu, R., Liu, Z., Sheng, X., Zhang, Y., Liu, Y., Mu, Y., Zhu, Y., Sun, E., Zhang, J., Li, F., Xia, C., Ge, J., Liu, Z., Bu, Z., Zhao, D. :
A genome-wide CRISPR/Cas9 knockout screen identifies TMEM239 as an important host factor in facilitating African swine fever virus entry into early endosomes. PLoS Pathog 20:e1012256, 2024. Pubmed reference: 39024394. DOI: 10.1371/journal.ppat.1012256.
Zheng, Z., Xu, L., Dou, H., Zhou, Y., Feng, X., He, X., Tian, Z., Song, L., Gao, Y., Mo, G., Hu, J., Zhao, H., Wei, H., Church, G.M., Yang, L. :
Testing multiplexed anti-ASFV CRISPR-Cas9 in reducing African swine fever virus. Microbiol Spectr 12:e0216423, 2024. Pubmed reference: 38563791. DOI: 10.1128/spectrum.02164-23.
2023 Feng, W., Zhou, L., Zhao, P., Du, H., Diao, C., Zhang, Y., Liu, Z., Jin, W., Yu, J., Han, J., Okoth, E., Mrode, R., Liu, J.F. :
Comparative genomic analysis of warthog and Sus scrofa identifies adaptive genes associated with African swine fever. Biology (Basel) 12:1001, 2023. Pubmed reference: 37508430. DOI: 10.3390/biology12071001.
2022 Tu, C.F., Chuang, C.K., Yang, T.S. :
The application of new breeding technology based on gene editing in pig industry - A review. Anim Biosci 35:791-803, 2022. Pubmed reference: 34991204. DOI: 10.5713/ab.21.0390.
2021 Penrith, M.L., Bastos, A., Chenais, E. :
With or without a Vaccine-A review of complementary and alternative approaches to managing African swine fever in resource-constrained smallholder settings. Vaccines (Basel) 9:116, 2021. Pubmed reference: 33540948. DOI: 10.3390/vaccines9020116.
2020 McCleary, S., Strong, R., McCarthy, R.R., Edwards, J.C., Howes, E.L., Stevens, L.M., Sánchez-Cordón, P.J., Núñez, A., Watson, S., Mileham, A.J., Lillico, S.G., Tait-Burkard, C., Proudfoot, C., Ballantyne, M., Whitelaw, C.B.A., Steinbach, F., Crooke, H.R. :
Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus. Sci Rep 10:8951, 2020. Pubmed reference: 32488046. DOI: 10.1038/s41598-020-65808-1.
2019 Zhao, J., Lai, L., Ji, W., Zhou, Q. :
Genome editing in large animals: current status and future prospects. Natl Sci Rev 6:402-420, 2019. Pubmed reference: 34691891. DOI: 10.1093/nsr/nwz013.
2017 Popescu, L., Gaudreault, N.N., Whitworth, K.M., Murgia, M.V., Nietfeld, J.C., Mileham, A., Samuel, M., Wells, K.D., Prather, R.S., Rowland, R.R.R. :
Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1. Virology 501:102-106, 2017. Pubmed reference: 27898335. DOI: 10.1016/j.virol.2016.11.012.
2016 Lillico, S.G., Proudfoot, C., King, T.J., Tan, W., Zhang, L., Mardjuki, R., Paschon, D.E., Rebar, E.J., Urnov, F.D., Mileham, A.J., McLaren, D.G., Whitelaw, C.B. :
Mammalian interspecies substitution of immune modulatory alleles by genome editing. Sci Rep 6:21645, 2016. Pubmed reference: 26898342. DOI: 10.1038/srep21645.
2011 Palgrave, C.J., Gilmour, L., Lowden, C.S., Lillico, S.G., Mellencamp, M.A., Whitelaw, C.B. :
Species-specific variation in RELA underlies differences in NF-κB activity: a potential role in African swine fever pathogenesis. J Virol 85:6008-14, 2011. Pubmed reference: 21450812. DOI: 10.1128/JVI.00331-11.
2003 Sánchez-Torres, C., Gómez-Puertas, P., Gómez-del-Moral, M., Alonso, F., Escribano, J.M., Ezquerra, A., Domínguez, J. :
Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection. Arch Virol 148:2307-23, 2003. Pubmed reference: 14648288. DOI: 10.1007/s00705-003-0188-4.

Edit History


  • Created by Imke Tammen2 on 11 Jan 2022
  • Changed by Imke Tammen2 on 18 Dec 2023
  • Changed by Imke Tammen2 on 06 Apr 2024
  • Changed by Imke Tammen2 on 01 Jul 2024