OMIA:002063-9823 : Hyperlipidaemia/atherosclerosis, APOE-related in Sus scrofa (pig) |
In other species: dog , rabbit
Categories: Homeostasis / metabolism phene
Links to possible relevant human trait(s) and/or gene(s) in OMIM: 107741 (gene) , 617347 (trait)
Single-gene trait/disorder: yes
Disease-related: unknown
Key variant known: yes
Year key variant first reported: 2017
Species-specific description: This trait results from genetic modification via CRISPR/cas9: the affected animals are genetically-modified organisms (GMO).
Molecular basis: Fang et al. (2018): "clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 system (CRISPR/Cas9) was used to disrupt the ApoE gene in Bama miniature pigs. Biallelic-modified ApoE pigs with in-frame mutations (ApoEm/m ) and frameshift mutations (ApoE-/- ) were simultaneously produced. ApoE-/- pigs exhibited moderately increased plasma cholesterol levels when fed with a regular chow diet, but displayed severe hypercholesterolemia and spontaneously developed human-like atherosclerotic lesions in the aorta and coronary arteries after feeding on a high-fat and high-cholesterol (HFHC) diet for 6 months. Thus, these ApoE-/- pigs could be valuable large animal models for providing further insight into translational studies of atherosclerosis."
Genetic engineering:
Yes - variants have been created artificially, e.g. by genetic engineering or gene editing
Have human generated variants been created, e.g. through genetic engineering and gene editing
Associated gene:
| Symbol | Description | Species | Chr | Location | OMIA gene details page | Other Links |
|---|---|---|---|---|---|---|
| APOE | apolipoprotein E | Sus scrofa | 6 | NC_010448.4 (51373113..51375333) | APOE | Homologene, Ensembl , NCBI gene |
Cite this entry
Nicholas, F. W., Tammen, I., & Sydney Informatics Hub. (2023). OMIA:002063-9823: Online Mendelian Inheritance in Animals (OMIA) [dataset]. https://omia.org/. https://doi.org/10.25910/2AMR-PV70
References
Note: the references are listed in reverse chronological order (from the most recent year to the earliest year), and alphabetically by first author within a year.
| 2021 | Zhang, J., Khazalwa, E.M., Abkallo, H.M., Zhou, Y., Nie, X., Ruan, J., Zhao, C., Wang, J., Xu, J., Li, X., Zhao, S., Zuo, E., Steinaa, L., Xie, S. : |
| The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research. J Genet Genomics 48:347-360, 2021. Pubmed reference: 34144928. DOI: 10.1016/j.jgg.2021.03.015. | |
| Zhang, Y., Fatima, M., Hou, S., Bai, L., Zhao, S., Liu, E. : | |
| Research methods for animal models of atherosclerosis (Review). Mol Med Rep 24:871, 2021. Pubmed reference: 34713295. DOI: 10.3892/mmr.2021.12511. | |
| 2018 | Fang, B., Ren, X., Wang, Y., Li, Z., Zhao, L., Zhang, M., Li, C., Zhang, Z., Chen, L., Li, X., Liu, J., Xiong, Q., Zhang, L., Jin, Y., Liu, X., Li, L., Wei, H., Yang, H., Li, R., Dai, Y. : |
| Apolipoprotein E deficiency accelerates atherosclerosis development in miniature pigs. Dis Model Mech 11:dmm036632, 2018. Pubmed reference: 30305304. DOI: 10.1242/dmm.036632. | |
| 2017 | Huang, L., Hua, Z., Xiao, H., Cheng, Y., Xu, K., Gao, Q., Xia, Y., Liu, Y., Zhang, X., Zheng, X., Mu, Y., Li, K. : |
| CRISPR/Cas9-mediated ApoE-/- and LDLR-/- double gene knockout in pigs elevates serum LDL-C and TC levels. Oncotarget 8:37751-37760, 2017. Pubmed reference: 28465483. DOI: 10.18632/oncotarget.17154. |
Edit History
- Created by Frank Nicholas on 30 May 2019
- Changed by Frank Nicholas on 30 May 2019
- Changed by Imke Tammen2 on 25 Jun 2021
- Changed by Imke Tammen2 on 03 Nov 2021
- Changed by Imke Tammen2 on 18 Dec 2023