OMIA 002359-9823 : Resistance/susceptibility to classical swine fever virus (CSFV) in Sus scrofa

Possibly relevant human trait(s) and/or gene(s) (MIM number): 607810 (gene)

Mendelian trait/disorder: unknown

Considered a defect: yes

Species-specific description: Xie et al. (2028): "antiviral small hairpin RNAs (shRNAs) were selected and then inserted at the porcine Rosa26 (pRosa26) locus via a CRISPR/Cas9-mediated knock-in strategy. Finally, anti-CSFV transgenic (TG) pigs were produced by somatic nuclear transfer (SCNT). Notably, in vitro and in vivo viral challenge assays further demonstrated that these TG pigs could effectively limit the replication of CSFV and reduce CSFV-associated clinical signs and mortality, and disease resistance could be stably transmitted to the F1-generation."

Xie et al. (2020): "The RSAD2 exhibits antiviral activity against various DNA and RNA viruses. In this study, we successfully accomplished site-specific insertion of the porcine RSAD2 gene (pRSAD2) at the porcine ROSA26 (pROSA26) locus, generating pRSAD2 gene knock-in (pRSAD2-KI) PK-15 cells and porcine foetal fibroblasts (PFFs) via CRISPR/Cas9 technology. ... More importantly, we ultimately successfully produced a pRSAD2-KI pig that constitutively overexpressed the pRSAD2, viral challenge results indicated that fibroblasts isolated from the pRSAD2-KI pig reduced CSFV infection." These studies used genetically modified organisms (GMO).

Associated gene:

Symbol Description Species Chr Location OMIA gene details page Other Links
RSAD2 radical S-adenosyl methionine domain containing 2 Sus scrofa 3 NC_010445.4 (128897809..128880853) RSAD2 Homologene, Ensembl, NCBI gene

References


Note: the references are listed in reverse chronological order (from the most recent year to the earliest year), and alphabetically by first author within a year.
2021 Zhang, J., Khazalwa, E.M., Abkallo, H.M., Zhou, Y., Nie, X., Ruan, J., Zhao, C., Wang, J., Xu, J., Li, X., Zhao, S., Zuo, E., Steinaa, L., Xie, S. :
The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research. J Genet Genomics 48:347-360, 2021. Pubmed reference: 34144928. DOI: 10.1016/j.jgg.2021.03.015.
2020 Xie, Z., Jiao, H., Xiao, H., Jiang, Y., Liu, Z., Qi, C., Zhao, D., Jiao, S., Yu, T., Tang, X., Pang, D., Ouyang, H. :
Generation of pRSAD2 gene knock-in pig via CRISPR/Cas9 technology. Antiviral Res 174:104696, 2020. Pubmed reference: 31862502. DOI: 10.1016/j.antiviral.2019.104696.
2018 Xie, Z., Pang, D., Yuan, H., Jiao, H., Lu, C., Wang, K., Yang, Q., Li, M., Chen, X., Yu, T., Chen, X., Dai, Z., Peng, Y., Tang, X., Li, Z., Wang, T., Guo, H., Li, L., Tu, C., Lai, L., Ouyang, H. :
Genetically modified pigs are protected from classical swine fever virus. PLoS Pathog 14:e1007193, 2018. Pubmed reference: 30543715. DOI: 10.1371/journal.ppat.1007193.

Edit History


  • Created by Imke Tammen2 on 25 Jun 2021
  • Changed by Imke Tammen2 on 25 Jun 2021