OMIA 002462-9823 : Disease resistance, beta defensin-related in Sus scrofa

Mendelian trait/disorder: unknown

Considered a defect: unknown

Species-specific name: Huang et al. (2020) “aimed to generate marker-free pbd-2 knock-in pigs using the CRISPR/Cas9 and Cre/loxP systems. Two copies of pbd-2 gene linked by a T2A sequence were inserted into the porcine Rosa26 locus through CRISPR/Cas9-mediated homology-directed repair. The floxed selectable marker gene neoR, used for G418 screening of positive cell clones, was removed by cell-penetrating Cre recombinase with a recombination efficiency of 48.3%. Cloned piglets were produced via somatic cell nuclear transfer and correct insertion of pbd-2 genes was confirmed by PCR and Southern blot. Immunohistochemistry and immunofluorescence analyses indicated that expression levels of PBD-2 in different tissues of transgenic (TG) piglets were significantly higher than those of their wild-type (WT) littermates. Bactericidal assays demonstrated that there was a significant increase in the antimicrobial properties of the cell culture supernatants of porcine ear fibroblasts from the TG pigs in comparison to those from the WT pigs.” This phene includes references to studies involving genetically modified organisms (GMO).

Reference


2020 Huang, J., Wang, A., Huang, C., Sun, Y., Song, B., Zhou, R., Li, L. :
Generation of marker-free pbd-2 knock-in pigs using the CRISPR/Cas9 and Cre/loxP systems. Genes (Basel) 11:951, 2020. Pubmed reference: 32824735. DOI: 10.3390/genes11080951.

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  • Created by Imke Tammen2 on 26 Oct 2021