OMIA:002660-9031 : Resistance/susceptibility to avian influenza virus in Gallus gallus (chicken)

In other species: Mallard , pig

Categories: Immune system phene

Mendelian trait/disorder: no

Mode of inheritance: Multifactorial

Considered a defect: yes

Key variant known: no

Species-specific description: Byun et al. (2017) "used recombinant lentiviruses to generate transgenic [TG] chickens expressing the 3D8 scFv gene under the control of the chicken β-actin promoter. ... [Their] results suggest that the transgenic chickens developed in this study could be useful for controlling potential within-flock AIV transmission." Yang et al. (2023) compared various parameters in non-TG and TG offspring female chickens: "Some serum parameters and cytokines were significantly different ... . Despite these differences, the mortality rates, body weight, egg production rates, and egg weight were not significantly different in the experimental groups of non-TG and TG offspring female chickens ... ." Idoko-Akoh et al. (2023) "In chickens, influenza A virus (IAV) relies on host protein ANP32A. Here we use CRISPR/Cas9 to generate homozygous gene edited (GE) chickens containing two ANP32A amino acid substitutions ... . After IAV challenge, 9/10 edited chickens remain uninfected. Challenge with a higher dose, however, led to breakthrough infections. ... Additional genome editing for removal of ANP32B and ANP32E eliminated all viral growth in chicken cells. Our data illustrate a first proof of concept step to generate IAV-resistant chickens and show that multiple genetic modifications will be required to curtail viral escape." This phene includes references to studies involving gene edited or genetically modified organisms (GMO).

Genetic engineering: Yes - in addition to the occurrence of natural variants, variants have been created artificially, e.g. by genetic engineering or gene editing
Have human generated variants been created, e.g. through genetic engineering and gene editing

Associated genes:

Symbol Description Species Chr Location OMIA gene details page Other Links
ANP32A acidic (leucine-rich) nuclear phosphoprotein 32 family, member A Gallus gallus 10 NC_052541.1 (19284020..19264398) ANP32A Homologene, Ensembl , NCBI gene
ANP32B acidic (leucine-rich) nuclear phosphoprotein 32 family, member B Gallus gallus 28 NC_052559.1 (2152928..2161666) ANP32B Homologene, Ensembl , NCBI gene
ANP32E acidic (leucine-rich) nuclear phosphoprotein 32 family, member E Gallus gallus 25 NC_052556.1 (246003..224227) ANP32E Homologene, Ensembl , NCBI gene

Cite this entry

Nicholas, F. W., Tammen, I., & Sydney Informatics Hub. (2023). OMIA:002660-9031: Online Mendelian Inheritance in Animals (OMIA) [dataset].


Note: the references are listed in reverse chronological order (from the most recent year to the earliest year), and alphabetically by first author within a year.

2023 Bertram, H., Wilhelmi, S., Rajavel, A., Boelhauve, M., Wittmann, M., Ramzan, F., Schmitt, A.O., Gültas, M. :
Comparative investigation of coincident single nucleotide polymorphisms underlying avian influenza viruses in chickens and ducks. Biology (Basel) 12:969, 2023. Pubmed reference: 37508399. DOI: 10.3390/biology12070969.
Bryson, K.J., Sives, S., Lee, H.M., Borowska, D., Smith, J., Digard, P., Vervelde, L. :
Comparative analysis of different inbred chicken lines highlights how a hereditary inflammatory state affects susceptibility to avian influenza virus. Viruses 15:591, 2023. Pubmed reference: 36992300. DOI: 10.3390/v15030591.
Cohen, J. :
In 'proof of concept,' CRISPR-edited chickens shrug off flu. Science 382:140-141, 2023. Pubmed reference: 37824660. DOI: 10.1126/science.adl2973.
Idoko-Akoh, A., Goldhill, D.H., Sheppard, C.M., Bialy, D., Quantrill, J.L., Sukhova, K., Brown, J.C., Richardson, S., Campbell, C., Taylor, L., Sherman, A., Nazki, S., Long, J.S., Skinner, M.A., Shelton, H., Sang, H.M., Barclay, W.S., McGrew, M.J. :
Creating resistance to avian influenza infection through genome editing of the ANP32 gene family. Nat Commun 14:6136, 2023. Pubmed reference: 37816720. DOI: 10.1038/s41467-023-41476-3.
Yang, H., Ock, S.A., Lee, S., Park, M.R., Kim, S., No, J., Oh, K.B., Wi, H., Jung, S.K., Jo, Y.J., Lee, B.R., Lee, M., Byun, S.J. :
Mortality, growth, and egg production do not differ between nontransgenic and transgenic female chickens with ubiquitous expression of the 3D8 single chain variable fragment gene. Poult Sci 102:102802, 2023. Pubmed reference: 37307631. DOI: 10.1016/j.psj.2023.102802.
2018 Drobik-Czwarno, W., Wolc, A., Fulton, J.E., Jankowski, T., Arango, J., O'Sullivan, N.P., Dekkers, J.C.M. :
Genetic basis of resistance to avian influenza in different commercial varieties of layer chickens. Poult Sci 97:3421-3428, 2018. Pubmed reference: 29924353. DOI: 10.3382/ps/pey233.
2017 June Byun, S., Yuk, S.S., Jang, Y.J., Choi, H., Jeon, M.H., Erdene-Ochir, T.O., Kwon, J.H., Noh, J.Y., Sun Kim, J., Gyu Yoo, J., Song, C.S. :
Transgenic chickens expressing the 3D8 single chain variable fragment protein suppress avian influenza transmission. Sci Rep 7:5938, 2017. Pubmed reference: 28724948. DOI: 10.1038/s41598-017-05270-8.
2016 Ruiz-Hernandez, R., Mwangi, W., Peroval, M., Sadeyen, J.R., Ascough, S., Balkissoon, D., Staines, K., Boyd, A., McCauley, J., Smith, A., Butter, C. :
Host genetics determine susceptibility to avian influenza infection and transmission dynamics. Sci Rep 6:26787, 2016. Pubmed reference: 27279280. DOI: 10.1038/srep26787.

Edit History

  • Created by Imke Tammen2 on 31 Mar 2023
  • Changed by Imke Tammen2 on 31 Mar 2023
  • Changed by Imke Tammen2 on 15 Jun 2023
  • Changed by Imke Tammen2 on 15 Oct 2023
  • Changed by Imke Tammen2 on 18 Dec 2023