OMIA:002063-9823 : Hyperlipidaemia/atherosclerosis, APOE-related in Sus scrofa
In other species: rabbit , dog
Categories: Homeostasis / metabolism phene
Possibly relevant human trait(s) and/or gene(s)s (MIM numbers): 107741 (gene) , 617347 (trait)
Links to MONDO diseases: No links.
Mendelian trait/disorder: yes
Considered a defect: unknown
Key variant known: yes
Year key variant first reported: 2017
Species-specific description: This trait results from genetic modification via CRISPR/cas9: the affected animals are genetically-modified organisms (GMO).
Molecular basis: Fang et al. (2018): "clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 system (CRISPR/Cas9) was used to disrupt the ApoE gene in Bama miniature pigs. Biallelic-modified ApoE pigs with in-frame mutations (ApoEm/m ) and frameshift mutations (ApoE-/- ) were simultaneously produced. ApoE-/- pigs exhibited moderately increased plasma cholesterol levels when fed with a regular chow diet, but displayed severe hypercholesterolemia and spontaneously developed human-like atherosclerotic lesions in the aorta and coronary arteries after feeding on a high-fat and high-cholesterol (HFHC) diet for 6 months. Thus, these ApoE-/- pigs could be valuable large animal models for providing further insight into translational studies of atherosclerosis."
|Symbol||Description||Species||Chr||Location||OMIA gene details page||Other Links|
|APOE||apolipoprotein E||Sus scrofa||6||NC_010448.4 (51373113..51375333)||APOE||Homologene, Ensembl , NCBI gene|
Note: the references are listed in reverse chronological order (from the most recent year to the earliest year), and alphabetically by first author within a year.
|2021||Zhang, J., Khazalwa, E.M., Abkallo, H.M., Zhou, Y., Nie, X., Ruan, J., Zhao, C., Wang, J., Xu, J., Li, X., Zhao, S., Zuo, E., Steinaa, L., Xie, S. :|
|The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research. J Genet Genomics 48:347-360, 2021. Pubmed reference: 34144928 . DOI: 10.1016/j.jgg.2021.03.015.|
|Zhang, Y., Fatima, M., Hou, S., Bai, L., Zhao, S., Liu, E. :|
|Research methods for animal models of atherosclerosis (Review). Mol Med Rep 24:871, 2021. Pubmed reference: 34713295 . DOI: 10.3892/mmr.2021.12511.|
|2018||Fang, B., Ren, X., Wang, Y., Li, Z., Zhao, L., Zhang, M., Li, C., Zhang, Z., Chen, L., Li, X., Liu, J., Xiong, Q., Zhang, L., Jin, Y., Liu, X., Li, L., Wei, H., Yang, H., Li, R., Dai, Y. :|
|Apolipoprotein E deficiency accelerates atherosclerosis development in miniature pigs. Dis Model Mech 11:, 2018. Pubmed reference: 30305304 . DOI: 10.1242/dmm.036632.|
|2017||Huang, L., Hua, Z., Xiao, H., Cheng, Y., Xu, K., Gao, Q., Xia, Y., Liu, Y., Zhang, X., Zheng, X., Mu, Y., Li, K. :|
|CRISPR/Cas9-mediated ApoE-/- and LDLR-/- double gene knockout in pigs elevates serum LDL-C and TC levels. Oncotarget 8:37751-37760, 2017. Pubmed reference: 28465483 . DOI: 10.18632/oncotarget.17154.|
- Changed by Frank Nicholas on 30 May 2019
- Created by Frank Nicholas on 30 May 2019
- Changed by Imke Tammen2 on 25 Jun 2021
- Changed by Imke Tammen2 on 03 Nov 2021