OMIA 002073-9913 : Resistance/susceptibility to Mannheimia haemolytica leukotoxin in Bos taurus

Mendelian trait/disorder: yes

Mode of inheritance: Autosomal Recessive

Considered a defect: no

Key variant known: yes

Year key variant first reported: 2016

Species-specific description: The resistant cattle described in this entry are genetically-modified organisms (GMO)

Molecular basis: As explained by Shanthalingam et al. (2016), "the signal peptide of CD18 [encoded by ITGB2], an adhesive protein expressed on the surface of leukocytes of cattle and other ruminants, is not cleaved. Intriguingly, leukotoxin produced by Mannheimia haemolytica binds to the intact signal peptide and causes lysis of ruminant leukocytes, resulting in acute pneumonia. In this proof-of-principle study, we used precise gene editing to induce a single amino acid substitution in CD18, which resulted in the cleavage of the signal peptide and abrogation of leukotoxin-induced cytolysis of leukocytes of the gene-edited bovine fetus. This report demonstrates the feasibility of developing lines of cattle genetically resistant to M. haemolytica-caused pneumonia, which will have significant impact on the sustainability of food-animal production in the United States and elsewhere. "

Associated gene:

Symbol Description Species Chr Location OMIA gene details page Other Links
ITGB2 integrin subunit beta 2 Bos taurus 1 NC_037328.1 (144788839..144759539) ITGB2 Homologene, Ensembl, NCBI gene

Reference


2016 Shanthalingam, S., Tibary, A., Beever, J.E., Kasinathan, P., Brown, W.C., Srikumaran, S. :
Precise gene editing paves the way for derivation of Mannheimia haemolytica leukotoxin-resistant cattle. Proc Natl Acad Sci U S A :, 2016. Pubmed reference: 27799556. DOI: 10.1073/pnas.1613428113.

Edit History


  • Created by Frank Nicholas on 09 Nov 2016
  • Changed by Frank Nicholas on 09 Nov 2016