OMIA:001990-9823 : Cancer, TP53-related in Sus scrofa |
In other species: crab-eating macaque , golden hamster
Categories: Neoplasm
Possibly relevant human trait(s) and/or gene(s)s (MIM numbers): 109800 (trait) , 114480 (trait) , 137215 (trait) , 601626 (trait) , 211980 (trait) , 260350 (trait) , 614470 (trait) , 202300 (trait) , 114500 (trait) , 114550 (trait) , 607107 (trait) , 259500 (trait) , 614740 (trait) , 137800 (trait) , 191170 (gene) , 190070 (gene) , 151623 (trait)
Links to MONDO diseases: No links.
Mendelian trait/disorder: unknown
Considered a defect: yes
Species-specific description: Genetically-modified organism; GMO
Molecular basis: Sieren et al. (2014) created pigs transgenic for the R167H variant of TP53, equivalent to the human variant R175H that is "one of the most frequent missense mutations in sporadic [human] tumors". Homozygotes for this variant "developed lymphomas and osteogenic tumors, recapitulating the tumor types observed in mice and humans expressing orthologous TP53 mutant alleles".
Schook et al. (2015) developed a line of pigs transgenic for the same TP53 variant and for another oncogenic variant, namely G12D in the KRAS gene.
Tanihara et al. (2018) "generated TP53-mutant pigs by gene editing via electroporation of the Cas9 protein (GEEP), a process that involves introducing the Cas9 protein and single-guide RNA (sgRNA) targeting exon 3 and intron 4 of TP53 into in vitro-fertilized zygotes. ... Our results indicated that the mutations caused by gene editing successfully induced tumor phenotypes in both TP53 mosaic- and bi-allelic mutant pigs."
Associated genes:
| Symbol | Description | Species | Chr | Location | OMIA gene details page | Other Links |
|---|---|---|---|---|---|---|
| TP53 | tumor protein p53 | Sus scrofa | 12 | NC_010454.4 (52953786..52939643) | TP53 | Homologene, Ensembl , NCBI gene |
| KRAS | Kirsten rat sarcoma viral oncogene homolog | Sus scrofa | 5 | NC_010447.5 (48508774..48546260) | KRAS | Homologene, Ensembl , NCBI gene |
References
Note: the references are listed in reverse chronological order (from the most recent year to the earliest year), and alphabetically by first author within a year.
| 2023 | Ghosn, M., Elsakka, A.S., Petre, E.N., Cheleuitte-Nieves, C., Tammela, T., Monette, S., Ziv, E., Schachtschneider, K.M., Srimathveeravalli, G., Yarmohammadi, H., Edward Boas, F., Solomon, S.B. : |
| Induction and preliminary characterization of neoplastic pulmonary nodules in a transgenic pig model. Lung Cancer 178:157-165, 2023. Pubmed reference: 36868176 . DOI: 10.1016/j.lungcan.2023.02.013. | |
| 2022 | Carlson, A.L., Carrazco-Carrillo, J., Loder, A., Elkhadragy, L., Schachtschneider, K.M., Padilla-Benavides, T. : |
| The Oncopig as an emerging model to investigate copper regulation in cancer. Int J Mol Sci 23:14012, 2022. Pubmed reference: 36430490 . DOI: 10.3390/ijms232214012. | |
| Elkhadragy, L., Dasteh Goli, K., Totura, W.M., Carlino, M.J., Regan, M.R., Guzman, G., Schook, L.B., Gaba, R.C., Schachtschneider, K.M. : | |
| Effect of CRISPR knockout of AXIN1 or ARID1A on proliferation and migration of porcine hepatocellular carcinoma. Front Oncol 12:904031, 2022. Pubmed reference: 35669430 . DOI: 10.3389/fonc.2022.904031. | |
| 2021 | Elkhadragy, L., Regan, M.R., M Totura, W., Goli, K.D., Patel, S., Garcia, K., Stewart, M., Schook, L.B., Gaba, R.C., Schachtschneider, K.M. : |
| Generation of genetically tailored porcine liver cancer cells by CRISPR/Cas9 editing. Biotechniques 70:37-48, 2021. Pubmed reference: 33222517 . DOI: 10.2144/btn-2020-0119. | |
| Jarvis, S., Koumadoraki, E., Madouros, N., Sharif, S., Saleem, A., Khan, S. : | |
| Non-rodent animal models of osteosarcoma: A review. Cancer Treat Res Commun 27:100307, 2021. Pubmed reference: 33453605 . DOI: 10.1016/j.ctarc.2021.100307. | |
| Li, H., Cheng, W., Chen, B., Pu, S., Fan, N., Zhang, X., Jiao, D., Shi, D., Guo, J., Li, Z., Qing, Y., Jia, B., Zhao, H.Y., Wei, H.J. : | |
| Efficient generation of P53 biallelic mutations in Diannan Miniature pigs using RNA-guided base editing. Life (Basel) 11:1417, 2021. Pubmed reference: 34947951 . DOI: 10.3390/life11121417. | |
| Rubinstein, C.D., McLean, D.T., Lehman, B.P., Meudt, J.J., Schomberg, D.T., Krentz, K.J., Reichert, J.L., Meyer, M.B., Adams, M., Konsitzke, C.M., Shanmuganayagam, D. : | |
| Assessment of mosaicism and detection of cryptic alleles in CRISPR/Cas9-Engineered Neurofibromatosis Type 1 and TP53 mutant porcine models reveals overlooked challenges in precision modeling of human diseases. Front Genet 12:721045, 2021. Pubmed reference: 34630515 . DOI: 10.3389/fgene.2021.721045. | |
| Segatto, N.V., Bender, C.B., Seixas, F.K., Schachtschneider, K., Schook, L., Robertson, N., Qazi, A., Carlino, M., Jordan, L., Bolt, C., Collares, T. : | |
| Perspective: Humanized pig models of bladder cancer. Front Mol Biosci 8:681044, 2021. Pubmed reference: 34079821 . DOI: 10.3389/fmolb.2021.681044. | |
| Tanihara, F., Hirata, M., Otoi, T. : | |
| Current status of the application of gene editing in pigs. J Reprod Dev 67:177-187, 2021. Pubmed reference: 33840678 . DOI: 10.1262/jrd.2021-025. | |
| Zhang, J., Khazalwa, E.M., Abkallo, H.M., Zhou, Y., Nie, X., Ruan, J., Zhao, C., Wang, J., Xu, J., Li, X., Zhao, S., Zuo, E., Steinaa, L., Xie, S. : | |
| The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research. J Genet Genomics 48:347-360, 2021. Pubmed reference: 34144928 . DOI: 10.1016/j.jgg.2021.03.015. | |
| 2018 | Tanihara, F., Hirata, M., Nguyen, N.T., Le, Q.A., Hirano, T., Takemoto, T., Nakai, M., Fuchimoto, D.I., Otoi, T. : |
| Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation. PLoS One 13:e0206360, 2018. Pubmed reference: 30352075 . DOI: 10.1371/journal.pone.0206360. | |
| 2017 | Shen, Y., Xu, K., Yuan, Z., Guo, J., Zhao, H., Zhang, X., Zhao, L., Qing, Y., Li, H., Pan, W., Jia, B., Zhao, H.Y., Wei, H.J. : |
| Efficient generation of P53 biallelic knockout Diannan miniature pigs via TALENs and somatic cell nuclear transfer. J Transl Med 15:224, 2017. Pubmed reference: 29100547 . DOI: 10.1186/s12967-017-1327-0. | |
| 2016 | Saalfrank, A., Janssen, K.P., Ravon, M., Flisikowski, K., Eser, S., Steiger, K., Flisikowska, T., Müller-Fliedner, P., Schulze, É., Brönner, C., Gnann, A., Kappe, E., Böhm, B., Schade, B., Certa, U., Saur, D., Esposito, I., Kind, A., Schnieke, A. : |
| A porcine model of osteosarcoma. Oncogenesis 5:e210, 2016. Pubmed reference: 26974205 . DOI: 10.1038/oncsis.2016.19. | |
| 2015 | Schook, L.B., Collares, T.V., Hu, W., Liang, Y., Rodrigues, F.M., Rund, L.A., Schachtschneider, K.M., Seixas, F.K., Singh, K., Wells, K.D., Walters, E.M., Prather, R.S., Counter, C.M. : |
| A genetic porcine model of cancer. PLoS One 10:e0128864, 2015. Pubmed reference: 26132737 . DOI: 10.1371/journal.pone.0128864. | |
| 2014 | Sieren, J.C., Meyerholz, D.K., Wang, X.J., Davis, B.T., Newell, J.D., Hammond, E., Rohret, J.A., Rohret, F.A., Struzynski, J.T., Goeken, J.A., Naumann, P.W., Leidinger, M.R., Taghiyev, A., Van Rheeden, R., Hagen, J., Darbro, B.W., Quelle, D.E., Rogers, C.S. : |
| Development and translational imaging of a TP53 porcine tumorigenesis model. J Clin Invest 124:4052-66, 2014. Pubmed reference: 25105366 . DOI: 10.1172/JCI75447. |
Edit History
- Created by Frank Nicholas on 13 Apr 2016
- Changed by Frank Nicholas on 14 Aug 2016
- Changed by Frank Nicholas on 08 Aug 2017
- Changed by Imke Tammen2 on 16 Jun 2021
- Changed by Imke Tammen2 on 25 Jun 2021
- Changed by Imke Tammen2 on 18 Sep 2021
- Changed by Imke Tammen2 on 26 Dec 2021
- Changed by Imke Tammen2 on 09 Jan 2022
- Changed by Imke Tammen2 on 11 Jan 2022
- Changed by Imke Tammen2 on 18 Jan 2023